Document Type : Original Research Article
Authors
1 Al-Amamien Al-Kadhimeen Medical City Baghdad, Iraq
2 Department of Microbiology, Al-Nahrain University,College of Medicine, Baghdad, Iraq
3 Department of Obstetrics & Gynecology AL-Nahrain University,College of Medicine, Baghdad, Iraq
Abstract
Background: The infection can present in newborns in different ways, ranging from no noticeable symptoms to more serious conditions like meningoencephalitis, myocarditis, and disseminated sepsis.
Methods: A cross-sectional study was conducted from November 2022 to June 2023. The study included 100 pregnant women with gestational ages ranging from 0 to 16 weeks, aged between 15 and 40 years. Each participant completed a standardized questionnaire. Afterward, placental tissue samples were collected from women who underwent elective termination of pregnancy. These samples were then analyzed for the presence of Coxsackievirus B (CVB) genome using a molecular biology approach. The detection process involved RNA extraction, cDNA, and qRT-PCR.
Results: A total of 100 women who had abortions were included in this study. Out of these, 88 had missed abortions, 10 had incomplete abortions, and 2 had spontaneous abortions. The mean ages of the women were as follows: 28±2.8 years for missed abortions, 29±1.5 years for incomplete abortions, and 32±0.5 years for spontaneous abortions. The mean gestational ages were 10±1.1 weeks for missed abortions, 10±1.5 weeks for incomplete abortions, and 14 weeks for spontaneous abortions. Our investigation found no presence of Coxsackie B virus nucleic acid in the placental tissues obtained from the women who had abortions. The internal control (quality control) and positive control both yielded positive results.
Conclusion: Despite the assay's high sensitivity for accurate detection, the study produced only null findings. These negative results are likely to be true negatives, indicating the absence of the target analyte.
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