Enter your email address
Submit
Write your message
Wed, May 25, 2022
[Archive]
Volume 7, Issue 4 (July & August 2022)
J Obstet Gynecol Cancer Res 2022, 7(4): 323-328 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Ostadian C, Mehrafza M, Eftekhari A, Aghajani S, Vahabzadeh H, Gholami M, et al . The Effect of Prolonged Incubation of Sperm at Testis Temperature versus Room Temperature on Semen Parameters- An Experimental Study. J Obstet Gynecol Cancer Res. 2022; 7 (4) :323-328
URL: http://jogcr.com/article-1-496-en.html
URL: http://jogcr.com/article-1-496-en.html
Caspian Ostadian1, Marzieh Mehrafza *2, Azadeh Eftekhari1, Shahrzad Aghajani1, Hajar Vahabzadeh1, Mahdis Gholami1, Azadeh Raoufi1, Sajedeh Samadnia1, Elmira Hosseinzadeh1, Ahmad Hosseini1
1- Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran
2- Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran , marzieh.mehrafza@gmail.com
2- Mehr Fertility Research Center, Guilan University of Medical Sciences, Rasht, Iran , marzieh.mehrafza@gmail.com
Abstract: (460 Views)
Background & Objective: The effect of storage time and temperature on the prepared semen sample was evaluated, but the optimal condition is unclear. The aim of this study was to assess the effect of long-term incubation of prepared sperm at testicular temperature versus room temperature on semen parameters and DNA fragmentation index (DFI).
Materials & Methods: Sperm samples were collected from 40 patients between 2019 and 2020. Each sample was separated into two parts and underwent a non-direct swim-up method. One group was placed in a 35°C incubator, and the other group was kept at room temperature (26°C) in the dark. Both groups were evaluated at intervals of 45 minutes, 24 hours and 48 hours after sampling in terms of sperm concentration, motility, morphology, and DFI. Student t-test and repeated measures analysis of variance were used.
Results: Sperm count (P=0.007) and motility (P<0.001) at 26°C in three-time intervals of 45 minutes, 24 hours and 48 hours were significantly higher than 35°C. The proportion of normal morphology spermatozoa at 26 and 35°C at 45 min, 24 h, and 48 h did not show a significant difference (P=0.08). DFI at 26°C in three-time intervals was significantly lower than 35°C (P=0.008).
Conclusion: The results of this study indicated that when the prepared sperm samples are incubated for 24 h at 26°C compared to 35°C, they show significantly better quality and good quality of sperm can be retained for several hours if stored at room temperature.
Systematic Review: Original Research |
Subject:
Reproductive Medicine
Received: 2021/09/13 | Accepted: 2021/11/23 | Published: 2022/03/14
Received: 2021/09/13 | Accepted: 2021/11/23 | Published: 2022/03/14
References
1. Ilacqua A, Izzo G, Emerenziani GP, Baldari C, Aversa A. Lifestyle and fertility: the influence of stress and quality of life on male fertility. Reprod Biol Endocrinol. 2018;16(1):115. [DOI:10.1186/s12958-018-0436-9] [PMID] [PMCID]
2. Ricci G, Perticarari S, Boscolo R, Montico M, Guaschino S, Presani G. Semen preparation methods and sperm apoptosis: swim-up versus gradient-density centrifugation technique. Fertil Steril. 2009;91(2):632-8. [DOI:10.1016/j.fertnstert.2007.11.068] [PMID]
3. Marı́n-Briggiler CI, Tezón JG, Miranda PV, Vazquez-Levin MH. Effect of incubating human sperm at room temperature on capacitation-related events. Fertil Steril. 2002;77(2):252-9. [DOI:10.1016/S0015-0282(01)02982-X]
4. Franken DR, van Wyk R, Stoumann C, Avari K. Temperature controlled centrifugation improves sperm retrieval. Andrologia. 2011;43(3):217-21. [DOI:10.1111/j.1439-0272.2010.01136.x] [PMID]
5. Thijssen A, Klerkx E, Huyser C, Bosmans E, Campo R, Ombelet W. Influence of temperature and sperm preparation on the quality of spermatozoa. Reprod Biomed Online. 2014;28(4):436-42. [DOI:10.1016/j.rbmo.2013.12.005] [PMID]
6. Boomsma CM, Heineman MJ, Cohlen BJ, Farquhar C. Semen preparation techniques for intrauterine insemination. Cochrane Database Syst Rev. 2004;17(3):CD004507. [DOI:10.1002/14651858.CD004507.pub2]
7. Bui AD, Sharma R, Henkel R, Agarwal A. Reactive oxygen species impact on sperm DNA and its role in male infertility. Andrologia. 2018;50(8):e13012. [DOI:10.1111/and.13012] [PMID]
8. White DR, Phillips DM, Bedford JM. Factors affecting the acrosome reaction in human spermatozoa. J Reprod Fertil. 1990;90(1):71-80. [DOI:10.1530/jrf.0.0900071] [PMID]
9. Jackson RE, Bormann CL, Hassun PA, Rocha AM, Motta EL, Serafini PC, et al. Effects of semen storage and separation techniques on sperm DNA fragmentation. Fertil Steril. 2010;94(7):2626-30. [DOI:10.1016/j.fertnstert.2010.04.049] [PMID]
10. Hosseini A, Khalili MA. Improvement of motility after culture of testicular spermatozoa: the effects of incubation timing and temperature. Transl Androl Urol. 2017;6(2):271-6. [DOI:10.21037/tau.2017.03.43] [PMID] [PMCID]
11. Çok T, Aytaç PÇ, Şimşek E, Haydardedeoğlu B, Kalaycı H, Özdemir H, et al. The effect of preserving prepared sperm samples at room temperature or at 37 C before intrauterine insemination (IUI) on clinical pregnancy rate. Turk J Obstet Gynecol. 2015;12(1):6-10. [DOI:10.4274/tjod.31644] [PMID] [PMCID]
12. Matsuura R, Takeuchi T, Yoshida A. Preparation and incubation conditions affect the DNA integrity of ejaculated human spermatozoa. Asian J Androl. 2010;12(5):753-9. [DOI:10.1038/aja.2010.46] [PMID] [PMCID]
13. Organization WH. WHO laboratory manual for the examination and processing of human semen. 2010.
14. Cohen J, Fehilly CB, Walters DE. Prolonged storage of human spermatozoa at room temperature or in a refrigerator. Fertil Steril. 1985;44(2):254-62. [DOI:10.1016/S0015-0282(16)48747-9]
15. Villarroya S, Scholler R. Lateral diffusion of a human sperm-head antigen during incubation in a capacitation medium and induction of the acrosome reaction in vitro. J Reprod Fertil. 1987;80(2):545-62. [DOI:10.1530/jrf.0.0800545] [PMID]
16. Osheroff JE, Visconti PE, Valenzuela JP, Travis AJ, Alvarez J, Kopf GS. Regulation of human sperm capacitation by a cholesterol efflux-stimulated signal transduction pathway leading to protein kinase A-mediated up-regulation of protein tyrosine phosphorylation. Mol Hum Reprod. 1999;5(11):1017-26. [DOI:10.1093/molehr/5.11.1017] [PMID]
17. Peer S, Eltes F, Berkovitz A, Yehuda R, Itsykson P, Bartoov B. Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37° C? Fertil Steril. 2007;88(6):1589-94. [DOI:10.1016/j.fertnstert.2007.01.069] [PMID]
18. Aboulmaouahib S, Madkour A, Kaarouch I, Saadani B, Sefrioui O, Louanjli N, et al. Effect of semen preparation technique and its incubation on sperm quality in the Moroccan population. Andrologia. 2017;49(6):1-7. [DOI:10.1111/and.12688] [PMID]
19. Petrella C, Hsieh J, Blake E, Thrift K, Zacur H, Zhao Y. Human sperm can survive at room temperature for weeks: Measured by motility and viability of sperm maintained under various conditions. Fertil Steril. 2003;80:210. [DOI:10.1016/S0015-0282(03)01468-7]
20. Lachaud C, Tesarik J, Canadas ML, Mendoza C. Apoptosis and necrosis in human ejaculated spermatozoa. Hum Reprod. 2004;19(3):607-10. [DOI:10.1093/humrep/deh130] [PMID]
21. Yavas Y, Selub MR. Intrauterine insemination (IUI) pregnancy outcome is enhanced by shorter intervals from semen collection to sperm wash, from sperm wash to IUI time, and from semen collection to IUI time. Fertil Steril. 2004;82(6):1638-47. [DOI:10.1016/j.fertnstert.2004.04.061] [PMID]
22. Fauque P, Lehert P, Lamotte M, Bettahar-Lebugle K, Bailly A, Diligent C, et al. Clinical success of intrauterine insemination cycles is affected by the sperm preparation time. Fertil Steril. 2014;101(6):1618-23. [DOI:10.1016/j.fertnstert.2014.03.015] [PMID]
23. Jansen CH, Elisen MG, Leenstra CW, Kaaijk EM, van Stralen KJ, Verhoeve HR. Longer time interval between semen processing and intrauterine insemination does not affect pregnancy outcome. Fertil Steril. 2017;108(5):764-9. [DOI:10.1016/j.fertnstert.2017.08.003] [PMID]
24. Ahmed I, Abdelateef S, Laqqan M, Amor H, Abdel‐Lah M, Hammadeh M. Influence of extended incubation time on human sperm chromatin condensation, sperm DNA strand breaks and their effect on fertilisation rate. Andrologia. 2018;50(4):1-8. [DOI:10.1111/and.12960] [PMID]
25. Nabi A, Khalili MA, Halvaei I, Roodbari F. Prolonged incubation of processed human spermatozoa will increase DNA fragmentation. Andrologia. 2014;46(4):374-9. [DOI:10.1111/and.12088] [PMID]
26. Aitken RJ. Free radicals, lipid peroxidation and sperm function. Reprod Fertil Dev. 1995;7(4):659-68. [DOI:10.1071/RD9950659] [PMID]
27. de Lamirande E, Jiang H, Zini A, Kodama H, Gagnon C. Reactive oxygen species and sperm physiology. Rev Reprod. 1997;2(1):48-54. [DOI:10.1530/ror.0.0020048] [PMID]
28. Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987;81(2):459-69. [DOI:10.1530/jrf.0.0810459] [PMID]
29. Tvrdá E, Arroyo F, Ďuračka M, López-Fernández C, Gosálvez J. Dynamic assessment of human sperm DNA damage II: the effect of sperm concentration adjustment during processing. J Assist Reprod Genet. 2019;36(4):799-807. [DOI:10.1007/s10815-019-01423-y] [PMID] [PMCID]
Send email to the article author
| Rights and permissions | |
 |
Journal of Obstetrics, Gynecology and Cancer Research by Farname Inc is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. Based on a work at http://jogcr.com/. |
Copyright Policy
Journal of Obstetrics, Gynecology and Cancer Research by Farname Inc is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Based on a work at http://jogcr.com/.
