Document Type : Original Research Article
Authors
- Zahra Shiravani 1
- Fatemehsadat Najib 1
- Mahvash Alirahimi 1
- Elham Askary 2
- Tahereh Poordast 2
- Nader Tanideh 3
- Shohreh Roozmeh 1
- Golsa Shekarkhar 4
- Sana Atbaei 1
- danilo porro 5
- Soudabeh Sabetian 6
- Claudia Cava 7
1 Obstetrics and Gynecology Oncology Division, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
2 Obstetrics and Gynecology Endoscopic Division, Department of Obstetrics and Gynecology, School of Medicine, Shiraz University of Medical Science, Shiraz, Iran
3 Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
4 Molecular Department, Deghat Pathology Laboratory, Tehran, Iran
5 NBFC, National Biodiversity Future Center, Palermo 90133, Italy
6 Infertility Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
7 Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, Segrate, 20090 Milan, Italy
Abstract
The endometriosis treatment was critical issue due to complications associated with current drug delivery system. Present investigation compared the curative effect of Vitamin D3 (VTD3) and Omega–3 (OG3) with Diphereline during the treatment of endometriosis. Endometriosis was induced in different groups containing 60 adult female rats. The rat model is categorized into 6 groups untreated and treated (Olive Oil (solvent), VTD3 (42 mcg/kg/day), OG3 (450 mg/kg/day), VTD3+OG3, Diphereline (3 mg/kg/day)). The suspension containing combination of Diphereline and supplements was injected and treated for 4 weeks to analyses the effect of supplements. The interleukin -6 (IL-6) and Tumor necrosis factor – alpha (TNFα) inflammatory responses were measured from the serum samples while endometrial implants was dissected and histopathological investigation was done. At the end of four weeks pathologic score was decreases significantly with simultaneous measurement of inflammation score of endometriotic lesion, size of implant area, IL-6, TNFα response and compared with untreated female rat. No significant different was observed in groups undergoing treatment of VTD3, OG3 and Diphereline. The combined effect of VTD3+OG3 has similar responses with Diphereline treated endometrial implants. In conclusion, treatment of VTD3 deficiency and making a change in dietary habits of high-risk population for endometriosis from adolescence may also play a preventative role in adulthood.
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