Document Type : Original Research Article
Authors
1 Center of Genetic Engineering, Al-Azhar University, Nasr City, Cairo, Egypt
2 Department of Obstetrics and Gynecology Faculty of Medicine, Ain Shams University, Cairo, Egypt.
3 Center of Genetic Engineering, Al-Azhar University, Nasr City, Cairo, Egypt.
Abstract
Background: Intracytoplasmic sperm injection (ICSI), a technique that involves an injection of a spermatozoon into the oocyte cytoplasm, has allowed the achievement of fertilization for a wide range of couples suffering from infertility.
Objective: The study assesses the influence of modified ICSI followed by chemically induced activation by calcimycin and Ionomycin in couples with a history of total fertilization failure (TFF).
Methods: A prospective analysis study was conducted with sibling oocytes to compare the application of calcimycin and Ionomycin after the M-ICSI technique. The study was conducted in a private IVF centre in Cairo, Egypt. A case with a TFF history assessed their fertilization rates, cleavage, and good-quality embryos on day three.
Results: 21 cycles comprised 134 oocytes treated with calcimycin and 125 oocytes treated with Ionomycin. Calcimycin-treated oocytes showed a fertilization rate of 39.5 %, a degeneration rate of 3.7 %, an arrest rate of 3.6 %, a cleavage rate of 45.5 %, and a good quality embryo rate of 37.1 %. On the other hand, ionomycin-treated oocytes showed a fertilization rate of 68.0 %, a degeneration rate of 6.0 %, an arrest rate of 3.7 %, a cleavage rate of 74.3 %, and a good quality embryo rate of 31.5 %. Ionomycin-treated oocytes had a significantly higher t-value of fertilization and cleavage rates than calcimycin-treated (p-value < 0.05).
Conclusion: M-ICSI followed by Ionomycin or ready-to-use calcimycin may treat TFF patients. Calcium increase could be achieved directly by mechanical activation and last longer by assisted oocyte activation (AOA) treatment.
Keywords
Main Subjects
)