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Volume 7, Issue 3 (May & June 2022)                   J Obstet Gynecol Cancer Res 2022, 7(3): 151-157 | Back to browse issues page

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Mousavi A, Akhavan S, Sabzi shahrbabaki F, Izadi-mood N, Yarandi F, Ghazimoghadam M, et al . Assessment of the Diagnostic Value of High-Risk HPV Molecular-based Methods for Triage of Iranian Women with Abnormal Cytological Findings of ASC-US and LSIL. J Obstet Gynecol Cancer Res. 2022; 7 (3) :151-157
URL: http://jogcr.com/article-1-437-en.html
1- Department of Gynecology-Oncology, Vali-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran , Azamsadat_mousavi@yahoo.com
2- Department of Gynecology-Oncology, Vali-e-Asr Hospital, Tehran University of Medical Sciences, Tehran, Iran
3- Department of Gynecology and Obstetrics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4- Department of Pathology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5- Department of Gynecology-Oncology, Yas Hospital, Tehran University of Medical Sciences, Tehran, Iran
6- Department of Pathology, Armin Pathobiology and Genetics Laboratory, Tehran, Iran
7- Department of Molecular Diagnosis, Armin Pathobiology and Genetics Laboratory, Tehran, Iran
8- Department of Gynecology and Obstetrics, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
9- Maternal, Fetal and Neonatal Research Center, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (536 Views)

Background & Objective:  Recent advances in molecular testing for human papillomavirus (HPV) has increased the accuracy of cervical screening programs. This study intended to estimate the diagnostic value of high-risk (HR) HPV DNA- and E6/E7 mRNA-based methods for triage of Iranian women with abnormal cytological results regarding the histopathological cut-off.
Materials & Methods: In this cross-sectional study, 360 non-pregnant women (≥ 21 years) who had faced abnormal cytological findings (ASC-US and LSIL) were enrolled and referred for further diagnostic tests. The INNO-LiPA® HPV Genotyping Extra-II and Aptima HPV assay kits were used in DNA- and E6/E7 mRNA-based methods for detection of HR-HPV. Regarding the CIN-2+ histopathological cut-off, the diagnostic value of each molecular-based assay was calculated.
Results: Among the study participants, 260 cases had ASC-US, and 100 had LSIL. The overall positivity rate for DNA- and mRNA-based methods was 74.4% (268/360) and 64.2% (231/360), respectively. Fifty-nine (16.4%) individuals showed CIN-2+. The DNA-based test showed higher sensitivity (100%) than the mRNA-based method (93.2%), while the mRNA-based method revealed greater clinical specificity (41.5%) compared to the DNA-based test (30.6%).
Conclusion: Our results revealed appropriate clinical sensitivity of the molecular-based methods for triage of Iranian women with abnormal cytological results; however, the mRNA-based method showed greater specificity for detection of CIN-2+.

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Systematic Review: Original Research | Subject: Gynecology Oncology
Received: 2021/06/29 | Accepted: 2021/08/13 | Published: 2022/01/12

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